Prediction of Neoplastic Transformation of Hydatidiform Mole: Current Evidence.

Gestational trophoblastic disease is not an uncommonly encountered pathology in clinical practice. The rate of post-molar neoplastic transformation is around 5-20% with higher rates after complete versus partial molar pregnancies. Recently, a role for molecular and genetic markers in the prediction of neoplastic transformation has emerged. We read with interest the article by St. Laurent et al. published in this issue of Reproductive Sciences. The authors compared miRNA profiles between complete hydatidiform moles (CHMs) and pre-gestational trophoblastic neoplasia CHM samples at three distinct tropho-miRNA clusters, 14q32, C19MC, and miR-371-3, as well as the expression of the contiguous DLK1, DIO3, and RTL1 genes. They found significant differences in expression of the 14q32 miRNA cluster and a fivefold decrease in protein expression of DIO3 but no difference in DIO3 mRNA expression. We reviewed the literature for similar studies looking at predictive tools for neoplastic transformation. We encourage future randomized controlled trials using these 2 novel risk predictors postulated by St. Laurent et al. to validate and guide future prophylactic chemotherapy for prevention of post-molar GTN.

NF-κB decoy polyplexes decrease P-glycoprotein-mediated multidrug resistance in colorectal cancer cells.

Multidrug resistance (MDR), a major cause for chemotherapy failure, has been linked to upregulation of ATP-dependent membrane efflux systems that limit intracellular accumulation of cytotoxic anticancer agents. P-glycoprotein (P-gp) encoded by the human ABCB1 gene was the first efflux transporter identified to contribute to MDR. ABCB1 gene expression is correlated with constitutive activation of the NF-κB signaling pathway in tumor cells. The objective of this research is to modulate P-gp activity in colon cancer cells using NF-κB decoy oligodeoxynucleotides (ODNs) that are effectively delivered into the nucleus of colorectal cancer cells by self-assembling nonviral nanoparticles comprising the novel poly[N-(2-hydroxypropyl)methacrylamide]-poly(N,N-dimethylaminoethylmethacrylate) diblock copolymer (pHPMA-b-pDMAEMA). Ethidium bromide intercalation and gel retardation assays demonstrated high DNA condensation capacity of pHPMA-b-pDMAEMA. Nanoparticles prepared with and without decoy ODNs did not significantly compromise cellular safety at N/P ratios ⩽4. Transfection efficiency of pHPMA-b-pDMAEMA polyplexes (N/P=4) in Caco-2 cells was comparable to TurboFect transfection standard, resulting in a 98% reduction in P-gp protein levels. As a pharmacodynamic consequence, intracellular accumulation of the P-gp substrate Rhodamine123 significantly increased by almost twofold. In conclusion, NF-κB ODN polyplexes fabricated with pHPMA-b-pDMAEMA polymer effectively reduced P-gp-mediated efflux activity in Caco-2 cells, suggesting successful interference with NF-κB-binding sites in the promoter region of the ABCB1 gene.

Correlation and path coefficient analyses in sweet melon (Cucumis melo var. Aegyptiacus L.) under irrigated and drought conditions.

Genotypic correlation and path analyses were carried out for growth, yield and fruit quality traits in 13 sweet melon genotypes collected from different places in Egypt. Seeds of these melon populations were sown under irrigated and drought stress conditions. The analysis of variance for the studied traits showed that the differences among genotypes were highly significant for all studied traits under irrigation and drought stress. Under irrigated conditions, total yield per plant was positively and significantly correlated with fruit weight, flesh fruit thickness and fruit length. Positive direct effects were exhibited for fruit weight, number of fruits per plant and stem length on total yield per plant, while maximum positive indirect effects on total yield per plant were exhibited by fruit length and flesh fruit thickness through fruit weight. In case of drought stress conditions, total yield per plant had the highest positive and significant correlation with fruit weight followed by flesh fruit thickness, fruit length and stem length. Fruit weight had the greatest positive direct effect on total yield per plant followed by number of fruits per plant, fruit length and total soluble solid content. Flesh fruit thickness and fruit length had high positive indirect effect on total yield per plant via fruit weight. The results obtained from correlation and path analyses showed that the efficiency in the selection for total yield per plant in sweet melon should increased through the selection of fruit weight under irrigated conditions and fruit weight and fruit length under drought conditions.

Oral lesions associated with HIV/AIDS in HIV-seropositive patients attending a counselling and treatment centre in Dar es Salaam.

OBJECTIVE: To assess the prevalences and patterns of oral lesions occurring in human immunodeficiency virus (HIV) and acquired immune deficiency syndrome (AIDS). METHODS: A cross-sectional study was conducted among 200 people living with HIV/AIDS (PlwHA) who regularly attended a counselling and treatment centre in Dar es Salaam, Tanzania. A questionnaire-guided interview and clinical oral assessment were used. Strict confidentiality and adherence to ethical codes were observed. RESULTS: The mean age of participants was 38.91 years (standard deviation: 10.424; mode: 35 years; median: 37.0 years; range: 15-76 years). Most participants (58.5%) were aware of predispositions towards the occurrence of oral lesions such as oral candidiasis (60.0%) in HIV/AIDS and most of these (72.0%) were aware that the lesions are treatable. Some participants reported occurrences of oral thrush (22.5%) and lip ulcerations (28.5%), although only 47.0% of these had sought medical advice. Examinations revealed that 29.0% of participants had at least one oral lesion associated with HIV/AIDS. Prevalences of the various types of lesion were: 11.5% for herpes simplex; 7.5% for oral candidiasis; 4.0% for oral hairy leukoplakia; 3.5% for Kaposi's sarcoma; 1.5% for dry mouth; 0.5% for angular cheilitis, and 0.5% for acute necrotising ulcerative gingivitis. Herpes simplex and Kaposi's sarcoma were more frequently observed in males (56.5% and 71.4%, respectively), whereas oral candidiasis and dry mouth were observed more often in females (86.7% and 66.7%, respectively) (χ(2) = 16.692, P = 0.016). CONCLUSIONS: Prevalences of oral lesions associated with HIV/AIDS in PlwHA and using antiretroviral therapy are persistent, of moderate intensity and vary according to individual immune status. These patients' level of awareness about oral lesions was satisfactory, but formal medicodental lines of management were not prioritised. Contemporary protocol for the management of oral lesions should be understood and disseminated to the general public by dentists.

Oral health experience during pregnancy and dental service utilization in Bariadi District, Tanzania.

A substantial proportion of pregnant women reports experiencing oral health problems during pregnancy. However, most of them perceive that such problems are normal in pregnancy and hence do not seek dentist consultation. The objective of this study was to determine the prenatal oral health experience and the utilization of dental care services among pregnant women attending reproductive and child health clinics in Bariadi District in Tanzania. Data was collected using a questionnaire-guided interview. Key variables were socio-demographic characteristics of pregnant women, oral health experience, and dental visits during pregnancy with reasons and treatment received. A total of 305 pregnant women (mean age=25.7 years) were involved in the study. Most of the listed oral health problems during pregnancy were reported by women with 2+ children. The frequent oral health problems among the pregnant women were bleeding gums (22.6%, N=69), pain in gums (21.6%, N=66), swollen gums (21.3%, N=65), dental pain (30.5, N=93), and tooth decay (25.6%, n=78). However, only 31.8% (N=97) visited a dental clinic for consultation most whom, were those with three or more children (χ²=.682; P=002). The pregnant women who had visited a dentist in the past 12 months were 11.1% (N=34), mostly those aged >24 years and those with informal employment (P<0.05). Curative and preventive treatments were received more significantly by the urban and with formal employment (P<0.01). In conclusion, pregnant women in Bariadi, Tanzania experiences substantial oral health problems for which they do not often utilize dentists for consultation and management during pregnancy. Dentists and other health workers should therefore, intensify dental screening, emphasizing active family and community participation as part of regular prenatal care.

Oral Health Experience during Pregnancy and Dental Service Utilization in Bariadi District; Tanzania

Abstract:A substantial proportion of pregnant women reports experiencing oral health problems during pregnancy. However; most of them perceive that such problems are normal in pregnancy and hence do not seek dentist consultation. The objective of this study was to determine the prenatal oral health experience and the utilization of dental care services among pregnant women attending reproductive and child health clinics in Bariadi District in Tanzania. Data was collected using a questionnaire-guided interview. Key variables were socio-demographic characteristics of pregnant women; oral health experience; and dental visits during pregnancy with reasons and treatment received. A total of 305 pregnant women (mean age=25.7 years) were involved in the study. Most of the listed oral health problems during pregnancy were reported by women with 2+ children. The frequent oral health problems among the pregnant women were bleeding gums (22.6; N=69); pain in gums (21.6; N=66); swollen gums (21.3; N=65); dental pain (30.5; N=93); and tooth decay (25.6; n=78). However; only 31.8 (N=97) visited a dental clinic for consultation most whom; were those with three or more children (?2=4.682; P=002). The pregnant women who had visited a dentist in the past 12 months were 11.1 (N=34); mostly those aged 24 years and those with informal employment (P0.05). Curative and preventive treatments were received more significantly by the urban and with formal employment (P0.01). In conclusion; pregnant women in Bariadi; Tanzania experiences substantial oral health problems for which they do not often utilize dentists for consultation and management during pregnancy. Dentists and other health workers should therefore; intensify dental screening; emphasizing active family and community participation as part of regular prenatal care

Cortisol and uncomplicated Plasmodium falciparum malaria in an area of unstable malaria transmission in eastern Sudan.

OBJECTIVE: To investigate the levels of serum cortisol in patients with uncomplicated Plasmodium falciparum (P. falciparum) malaria in an area of unstable malaria transmission in eastern Sudan. METHODS: The concentrations of cortisol were measured in sera of 25 patients with uncomplicated P. falciparum malaria (at presentation, 24 h and 7 d later) and 25 healthy volunteers using radioimmunoassay gamma counter. RESULTS: There was no significant difference in mean (SD) of total cortisol levels in patients with malaria in comparison with the control group; 602.2 (369.6) vs. 449.2(311.7) ng/mL, P=0.12. In patients with uncomplicated P. falciparum malaria, the mean (SD) presenting cortisol levels were significantly higher in comparison to the levels on day 7; 602.2 (369.6) vs. 373.6(139.1) ng/mL, P=0.009. In the patients with uncomplicated P. falciparum malaria (on presentation) cortisol levels were not correlated with initial temperature or the presenting parasitaemia. CONCLUSIONS: Thus, cortisol levels were not significantly different between the patients and the controls.

Oral health related knowledge, behaviours, attitude and self-assessed status of primary school teachers in Tanzania.

AIM: To assess the oral health related knowledge, attitudes, behaviours and self-assessed status of primary school teachers in Rungwe, Tanzania. SUBJECTS: 195 final year teacher trainees and 239 in-service teachers. METHOD: A structured questionnaire. RESULTS: School teachers were generally well informed and had moderate attitudes and behaviours to oral health related issues. However, a few but important deficiencies in this regard have been identified on which recommendations are presented. A substantial proportion of teachers reported having dental problems. However, the teachers had positive attitudes towards oral health education to pupils as part of a teaching curriculum. A professional support is called upon for teachers from the oral health personnel in terms of oral health education training at teachers' colleges, to prepare the teacher trainees for their future task. CONCLUSION: In-service teachers need to be motivated to improve their awareness on sound oral health information, attitudes and behaviours. This will facilitate their role as school oral health education providers in Tanzanian primary schools.

Sources of oral health information and teaching materials for primary schoolteachers in Rungwe District, Tanzania.

OBJECTIVE: To determine the various major sources of Oral Health Education (OHE) for primary school teachers in Rungwe District, Mbeya Region, Tanzania. DESIGN: Cross-sectional study using a self-administered close-ended questionnaire. PARTICIPANTS AND SETTING: 232 rural and urban in-service primary school teachers and 195 final-year-teacher trainees at college. MAIN OUTCOME MEASURES: Socio-demographic factors, common teachers' OHE sources, common sources of OHE teaching aides, and teachers' previous OHE training opportunity. RESULTS: Textbooks and a dental clinic were found to be the most common sources of OHE for a large proportion of in-service and trainee teachers, while neighbours and a teachers' college were reported by a smaller proportion. The District Dental Officer was the only significant source of oral health education teaching materials in this study. CONCLUSION AND RECOMMENDATION: While primary schoolteachers' sources for oral health education information was mainly textbooks and from a dental clinic, the source of teaching materials was largely a District Dental Officer. Details on the particular sources were not sought and further studies are therefore required. However, the findings highlight a need for standardised and equally distributed oral health material sources both for valid information and for teaching in schools.

Honey, a prospective antibiotic: extraction, formulation, and stability.

Old and recent reports show that honey has beneficial effects on the skin as antiseptic for wounds, burns and ulcers and as a healing promoter. Many investigators confirmed the usefulness of honey in the treatment of skin infections as well as internal diseases. The factors behind these effects are not completely explained. The aim of this study is: a) to investigate the antimicrobial activity of crude honey, b) to separate the fractions responsible for its activity, c) to formulate the honey extract as semisolid dosage forms, d) to study its release, and e) to determine its stability. The results showed that the ethylacetate honey extract showed antibacterial, anticandida and antifungal effects at low concentration. The release of honey extract from different ointment bases was depending on the constituents of the base, and its stability was found to be temperature and base dependent.

Leishmania major: in vitro ultrastructural study of the paraxial rod of promastigotes.

The flagellum of Leishmania major promastigotes has an intraflagellar structure known as the paraxial rod (PAR) which extends from a point halfway in the flagellar pocket to the tip of the flagellum, lying opposite the axonemal microtubule doublets 4-7. An expansion of the axonemal plasma membrane envelops the PAR and may provide desmosomal attachment at the orifice of the flagellar pocket. The complex organization of the 4-6 nm thick filaments in the PAR was studied by us in cross, oblique, longitudinal and tangential sections by electron microscope. These filaments are disposed in two parallel lamellae, one alongside the axoneme (ca. 45 nm thick), and the other alongside the plasma membrane (ca. 65 nm thick), with an interlamellar gap of about 22-28 nm. In each lamella, 8-12 parallel series of longitudinal filaments at ca. 30 nm intervals interdigitate with coplanar parallel series of oblique filaments at ca. 25 nm intervals and inclined to the long axis of the flagellum at ca. 48 degrees, and ca. 55 degrees, in the inner (paraxonemal) and outer lamella, respectively. The parallel filaments in each of the longitudinal and oblique series are spaced at ca. 8 nm intervals. They are cross-striated at ca. 30 nm intervals by transverse filaments which terminate occasionally on adjacent axonemal microtubules 5 and 6 in the inner lamella, and the plasma membrane in the outer lamella. Extending across the interlamellar gap is a set of parallel rows of 7-12 nearly parallel filaments at ca. 20 nm intervals. The part of the flagellar plasma membrane enclosing the PAR has a subplasmalemmal cytoskeleton consisting of a layer of longitudinal 2 nm filaments at 8 nm intervals, obliquely striated by parallel 2 nm filament doubles at ca. (-65) degrees with the long axis of the flagellum and ca. 20 nm periodicity. Each filament doublet stria apparently gives origin to collinear short filament doublet extensions that curve into juxtaposed meshes of the outer lamella. Microtubules of the axonemal doublets 5 and 6 are connected to electron-dense (ca. 12 nm thick) strips of the inner lamella of the PAR by longitudinal series of ca. 4 nm cross-links across a ca. 12 nm cleft.

Leishmania infecting man and wild animals in Saudi Arabia. 9. The black rat (Rattus rattus) a probable reservoir of visceral leishmaniasis in Gizan province, south-west Saudi Arabia.

Twenty-two black rats (Rattus rattus) were captured in houses where parasitologically confirmed cases of human visceral leishmaniasis had been recorded in Al-Arda Emara, Gizan province, south-west Saudi Arabia. Four of the rats were found to be infected with Leishmania; isoenzyme characterization showed that 3 were infected with L. donovani sensu lato zymodeme LON42 and the fourth with L. infantum zymodeme LON49. L. donovani s.l. LON42 has also been isolated from human visceral leishmaniasis patients living in this area, while dogs, but not humans, have been found to be infected with L. infantum LON49 in this part of Saudi Arabia.

Synthesis of some 2-(3,4-dimethoxy-phenyl)-1,3-propanediamines as potential dopaminergic agents.

The bilateral diamino analogs namely N,N'-dialkyl-N,N'-diaralkyl- and N,N,N',N'-tetraalkyl-2-(3,4-dimethoxyphenyl)-1,3-propanediamine s were synthesized. The biochemical determination of the brain levels of dopamine and norepinephrine as well as of brain monoamine oxidase (MAO) activity was performed.

The effect of cyclohexene, a preservative in dichloromethane on the liquid-liquid extraction and analysis of chlorinated drinking water.

Liquid-liquid extraction with dichloromethane is the Environmental Protection Agency's (EPA) method of choice for the analysis of acid or base neutral organic chemicals in water. Free radical chlorination and oxidation products of cyclohexene (the solvent preservative) in dichloromethane have been observed in extracts of chlorinated drinking water (after 10(5) fold concentration) by gas chromatography and gas chromatography-mass spectrometry. The chlorinated cyclohexene derivatives limit the quantitative and qualitative analysis of compounds eluting at Kovát's index less than 700-1000 and the use of these extracts for organoleptic and mutagenic studies. The effect of residual free chlorine in water on the cyclohexene present in the solvent was studied. Over ten cyclohexene derivatives were produced in every case when any residual free chlorine was present in the water. When chlorine is reduced to chloramine, the cyclohexene derivatives were greatly reduced in number and amount.

Pathogenicity of Crithidia fasciculata in the haemocoele of Glossina.

The mosquito flagellate Crithidia fasciculata produces intense haemocoelic infections following intrahaemocoelic inoculation into 5 species of Glossina--G. austeni, G. fuscipes fuscipes, G. morsitans morsitans, G. palpalis gambiensis and G. tachinoides. All Glossina inoculated with C. fasciculata died between days 4 and 9. Neither Trypanosoma brucei procyclics nor Leishmania hertigi promastigotes similarly inoculated into Glossina species, at the same dose, multiplied within the haemocoele and no deaths were recorded during the first 10 days post-injection. No mortalities amongst sham-injected controls occurred over the 10-day period.